林产化学与工业2005，Vol.25Issue(3)：63-66,4.
喜树种子中喜树碱和10-羟基喜树碱高效液相色谱法分析
DETERMINATION OF CAMPTOTHECIN AND 10-HYDROXYCAMPTOTHECIN IN CAMPTOTHECA ACUMINATA D. SEEDS BY HPLC
摘要
Abstract
A simple, rapid and sensitive method for the determination of contents of camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) in Camptotheca acuminata Decne. seeds by HPLC was established. The HPLC separation was performed on a KYA HIQ sil C18 column (250 mm×4.6 mm, 5 μm) at flow rate 1 mL/min, the solvent gradient program was as follow: acetonitrile-water increased linearly from 10 % to 40 % in the first 15 min, then decreased linearly to 10 % in 3 min; at time 21 min, stopped the program. The alkaloids were detected by a photodiode array detector and quantitatively analyzed at 254 nm for CPT and 266 nm for HCPT. CPT and HCPT were ultrasonically extracted by 60 % alcohol at 60 ℃ for 50 min. CPT and HCPT contents in different parts of C. acuminata D. seeds were determined by the above analyzing method. The results showed that CPT and HCPT levels were different in the four parts of C. acuminata D. seeds. CPT content was the highest in endosperm, lower in embryonic axis and cotyledon, and the lowest in seed coat, whereas HCPT content was the highest in endosperm, lower in seed coat, and the lowest in embryonic axis and cotyledon.关键词
喜树碱/10-羟基喜树碱/喜树种子/超声波提取Key words
camptothecin/10-hydroxycamptothecin/Camptotheca acuminata Decne. seeds/ultrasonic extraction分类
化学化工引用本文复制引用
王洋,阎秀峰,张玉红,于涛,马梅芳,魏焕勇..喜树种子中喜树碱和10-羟基喜树碱高效液相色谱法分析[J].林产化学与工业,2005,25(3):63-66,4.基金项目
国家自然科学基金资助项目(30070086) （30070086）
