中国林学（英文版）2006，Vol.8Issue(4)：63-68,6.
Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia 'Idaho'
Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia 'Idaho'
摘要
Abstract
Based on the plant regeneration system, a GUS gene transformation system to Idaho locust (Robinia pseudoacacia 'Idaho')mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots from the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of β-glucuronidase (GUS) and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% (5 out of 86 plants). With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southem blotting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R.pseudoacacia 'Idaho' mediated with A. tumefaciens.关键词
Robinia pseudoacacia 'Idaho'/Agrobacteriurn tumefaciens/GUS/gene transformationKey words
Robinia pseudoacacia 'Idaho'/Agrobacteriurn tumefaciens/GUS/gene transformation分类
农业科技引用本文复制引用
Sun Hai-jun,Li Min,Chen Shou-yi,He Si-jie,Wang Hua-fang..Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia 'Idaho'[J].中国林学（英文版）,2006,8(4):63-68,6.基金项目
The research was supported by the National High Technology and Development Program (Grant No.2004AA244040), by the National Research and Development Project in Transgenic Plants and Application (Grant No. JY03-B-29) and by the National Natural Science Foundation of China (Grant No.30371148). （Grant No.2004AA244040）
