分析化学2001，Vol.29Issue(6)：637-641,5.
批量法研究牛γ-球蛋白在尼龙亲和膜上的等温吸附行为
Study of Isothermal Adsorption of Bovine γGlobulin on Nylon Affinity Membrane with LTryptophane Ligand by Batch Method
摘要
Abstract
A new nylon affinity matrix with L-tryptoph ane as ligand was prepared for adsorption of bovine γ-globulin. Effects of tem
perature, ionic strength and pH on affinity adsorption of γ-globulin on affinity membrane were studied by batch method. The results show that the interaction between ligand and γ-globulin includes mainly electrostatic and hydrophobic interaction. The affinity adsorption at optimum condition obeys Langmuir adsorption model with maximum adsorption capacity and minimum nonspecific adsorption. The space location between protein and ligand and the configuration of proteins wi ll change when deviating from this condition.关键词
亲和膜色谱/L-色氨酸/牛γ-球蛋白/批量法引用本文复制引用
甘宏宇,商振华,刘学良,王俊德..批量法研究牛γ-球蛋白在尼龙亲和膜上的等温吸附行为[J].分析化学,2001,29(6):637-641,5.
