色谱2001，Vol.19Issue(1)：64-67,4.
毛细管电泳法测定淫羊藿甙的pKa值及其在中药淫羊藿中的含量
Determination of the pKa Value of Icariin and Its Content in the Chinese Herb Medicine Epimedium Grandiflorum Morr.by Capillary Electrophoresis
摘要
Abstract
Knowledge of dissociation constants is important for pred ictionand un derstanding the migration behavior of analytes in capillary electrophoresis. Icariin is the active com ponent of the Chinese herb medicine Epimedium grandiflorum Morr.. In order t o determine the dissociation constant of icariin and to show many important phar macology activities, a CZE method has been used to determine the ion mobility (μA-) and the pKa value of icariin based on the non -linear relation between μeff and[H+]and the linear relation between the reciprocal of effective mobility of the solute (1/μeff) and the [H+]of the buffer solution. In addition, the change of pKa o f icariin with the increase of ethanol concentration in the buffer was also inve stigated. Under the buffer condition of 24 mmol/L phosphate +30% ethanol, the c ontent of the active component icariin in Chinese herb Epimedium grandiflorum Morr. was quantitatively determined. The linear correlation equation was Y =6.96×10-3+17.0X, and the linear range is 0.032 g/L-0.354 g/L.关键词
毛细管电泳/淫羊藿甙/pKa值分类
化学化工引用本文复制引用
王德先,杨更亮,宋秀荣,寇素如..毛细管电泳法测定淫羊藿甙的pKa值及其在中药淫羊藿中的含量[J].色谱,2001,19(1):64-67,4.基金项目
河北省自然科学基金资助项目( （）
