中华医学杂志（英文版）2001，Vol.114Issue(1)：64-68,5.
CYP11B2基因在肝脏的表达及安体舒通对肝纤维化大鼠的治疗作用
Gene-CYP11B2 expression in rat liver in hepatic fibrogenesis induced by CCl4
摘要
Abstract
Objective To identify aldosterone synthase gene-CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone. Methods 160 Wistar rats weighing about 250?g were divided into 4 groups. In the model group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. In the antisterone group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. Antisterone equivalent to 20?mg*kg-1*d-1 was given intragastrically (ig). In the malotilate group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. Malotilate equivalent to 50?mg*kg-1*d-1 was given ig. In the control group (n=40), the rats were injected with olive oil only. After 2,4,6,8 and 10 weeks, animals were sacrificed, and morphological examination was carried out. The area of collagen was examined with an Image Analyse System. Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization. Results In situ hybridization and RT-PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred. Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks (P<0.05). There was no significant difference between the antisterone and malotilate groups (P>0.05). After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group (P<0.05). There was no significant difference between the antisterone and model groups (P>0.05). Conclusions The expression of CYP11B2 mRNA is up regulated in fibrotic liver. Antisterone can have a partial fibrogenesis-inhibiting effect in the early stages.关键词
CYP11B2 实验性肝纤维化肝星形细胞安体舒通原位杂交逆转录聚合酶链反应Key words
CYP11B2 gene/hepatic fibrogenesis/hepatic stellate cells/antisterone/in situ hybridization/reverse transcriptase polymerase chain reaction分类
医药卫生引用本文复制引用
李旭,杨希山,吴平生,孟莹,李淑梅,赖文岩..CYP11B2基因在肝脏的表达及安体舒通对肝纤维化大鼠的治疗作用[J].中华医学杂志（英文版）,2001,114(1):64-68,5.基金项目
The study was supported by the National Natural Science Foundation of China (No. 39870331). （No. 39870331）
