林业科学2001，Vol.37Issue(1)：64-71,8.
板栗主要栽培品种的分子鉴别
GENETIC ANALYSIS AND IDENTIFICATION OF MAIN CULTIVATED VARITIES OF CHESTNUT (CASTANEA MOLLISSIMA) BY RAPD MOLECULAR MARKERS
摘要
Abstract
The genetic diversity of chestnut was studied by RAPD molecular markers. The RAP D standardized procedure and the fingerprints database of chestnut were establis hed, which was the principal basement of chestnut breeding. The precise and reli able method identified the chestnut variety was supplied.With the 16 primers, 119 bands were produced. The 69 bands out of the 119 were p olymorphic(58%). The fingerprints database of 46 varieties was established and t he optimal combination of primers for these varieties identification was summari zed. It was shown that the four primers, out of the 16 primers which can produce d the polymorphic bands, could be used to distinguish the 46 varieties tested ef fectively. The optimal combimation of polymorphic loci was detected for variety discrimination. In order to summarize the better combination of primers, the gen etic distance matrix was calculated so as to indicate the particularity of prime rs combination.关键词
板栗/品种鉴别/RAPD分子标记/指纹图谱分类
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高捍东,黄宝龙..板栗主要栽培品种的分子鉴别[J].林业科学,2001,37(1):64-71,8.
