中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(11)：647-649,3.
氯喹衍生物CQ11逆转乳腺癌多药耐药细胞株MCF/DOX对多柔比星的耐药性
Reversal of CQ11, a chloroquine derivative,on multidrug resistance in doxorubicin-resistant breast cancer cell line MCF/DOX
摘要
Abstract
Objective: To investigate the reversal effect of CQ11, a chloroquine derivative, on multidrug resistance (MDR) in doxorubicin (DOX)-resistant human breast carcinoma cell line MCF/DOX. Methods: Cells of a human breast cancer cell line, MCF, and its DOX-resistant variant, MCF/DOX, were cultivated with DOX and/or CQ11. The cytotoxicity of drugs in vitro was assayed by MTT method. The accumulation of DOX in these cells was detected by fluorescence spectrophotometer. Results: MCF/DOX cells were 119 times more resistant to DOX in comparison with MCF cells. After simultaneous treatment with CQ11 at the concentrations of 1.0, 2.5 and 5.0 μmol/L, the IC50 of DOX for MCF/DOX cells decreased from 3.1±0.47 μmol/L to 0.58 ±0.032, 0.19±0.012 and 0.081±0.015 μmol/L, respectively, thus, increasing the DOX sensitivity by 5.3-fold (P<0.01), 16- fold (P < 0.01) and 38-fold (P < 0.01), respectively. In the accumulation assay of DOX, simultaneous incubation of MCF/DOX cells with CQ11 significantly increased the DOX accumulation in MCF/DOX cells. No such results were found in parental MCF cells. Conclusion: CQ11 had strong MDR reversal effect by enhancing intracellular DOX accumulation in MCF/DOX cells, indicating that CQ11 may be a promising MDR chemosensitivity.关键词
MDR/chloroquine/breast cancer/reversalKey words
MDR/chloroquine/breast cancer/reversal分类
医药卫生引用本文复制引用
Dalong Wu,Shirong Ma ,Fengying Sui ,Chengwen Zhang ,Lixin Yin ,Huanzhang Lu..氯喹衍生物CQ11逆转乳腺癌多药耐药细胞株MCF/DOX对多柔比星的耐药性[J].中德临床肿瘤学杂志（英文版）,2008,7(11):647-649,3.基金项目
grants from the National Natural Science Foundation of China (No. 39800181), the Research Foundation of Jiaxing Science and Technology Bureau (No. 2007AY2033) and the Research Foundation of Jiaxing College (No. 70107032). （No. 39800181）
