作物学报2002，Vol.28Issue(5)：650-653,4.
水稻RFLP连锁图谱的构建及控制小穗不育和花粉不育的QTL分析
Construction of RFLP Linkage Map and Identification of QTLs Controlling Spikelet and Pollen Sterility in Rice
摘要
Abstract
Quantitative trait locus (QTL) analysis has been carried out to identify genes conferring spikelet sterility and pollen sterility in rice. As a first step, an RFLP linkage map based on the recombinant inbred lines (RILs) was constructed. 125 RILs (F10) derived from a cross between a Japonica cultivar Taichung 65 and an Indica cultivar ARC10313 were developed. The RFLP map contained 113 well-dispersed RFLP markers. Total map length was 1462.4 cM Linkage arrangement of the RFLP markers was in good agreement with that of the previously constructed maps. 63 backcross F1 lines (BF1) derived from RILs were used as a segregating population for QTL analysis. Three QTLs for F1 spikelet sterility were detected on Chromosome 1, 7 and 11. Among them, a QTL near the RFLP marker R1789 (chromosome 7) was detected at significant level P=0.0001, and one QTL for F1 pollen sterility was also detected near R1789 marker. Rf4 on chromosome 7 is known as fertility restorer gene. Above QTLs detected on chromosome 7 in this study appeared to correspond to Rf4 locus.关键词
水稻/RFLP/小穗不育/花粉不育/QTLKey words
RFLP/Spikelet sterility/Pollen sterility/QTL analysis/Rice分类
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王春明,安井秀,吉村醇,翟虎渠,万建民..水稻RFLP连锁图谱的构建及控制小穗不育和花粉不育的QTL分析[J].作物学报,2002,28(5):650-653,4.基金项目
"教育部优秀骨干教师基金"和国家留学基金委员会资助. The study was supported in part by foundation for key teachers, ministry of education and conducted in Kyushu University, Japan during the visiting research by the first author supported by China Scholarsh （）
