中国临床康复2004，Vol.8Issue(29)：6519-6521,3.
肠缺血再灌注损伤对血清及脂肪组织Leptin水平的影响
Effect of intestinal ischemia-reperfusion injury on the leptin levels in serum and adipose tissue
摘要
Abstract
BACKGROUND: Recent studies suggest that adipose tissue is not only the reservation of fat, but also a multi-potential endocrine organ secreting many functional cytokines. Leptin is a protein specifically secreted by adipose tissue, and primarily reduces food-intake and promotes energy expenditure.OBJECTIVE: To explore the effect of intestinal ischemia-reperfusion injury on leptin levels in serum and adipose tissue, and find out the role of leptin playing in acute inflammatory responses.DESIGN: A completely random self-and mutual-control experiment.SETTING and MATERIALS: The experiment was collected and completed in Research Laboratory of Biochemistry, General Hospital of Chinese PLA.Three male New Zealand white rabbits and fifty-four male Sprague-Dawley rats were used for experimental use.INTERVENTIONS: New Zealand white rabbits were immunized to obtain anti-leptin, leptin antigen was iodinated by chloramines-T method, thus we established a concise radioimmunoassay for murine leptin. An intestinal ischemia-reperfusion injury model of rats was established, and rats were divided randomly into six groups: sham-operation group(sham), 60-minute ischemia and 30-minute reperfusion group(I60-R30), I60-R90, I60-R150,I60-R240 and I60-R360; each group contained nine rats.MAIN OUTCOME MEASURES: The changes of leptin concentrations in serum and adipose tissue before and after ischemia-reperfusion injury were checked by the radioimmunoassay.RESULTS: Compared with self-control(before injury), serum leptin level of I60-R30 decreased significantly(t=2.3891, P＜0.05), that of I60-R150 expressed a trend to increase and that of I60-R360 increased significantly ( t = - 2. 3437, P＜0.05 ). Compared with sham after injury [ (9.88±1.87)μg/L], serum leptin level of I60-R240 expressed a trend to increase, that of I60-R360[ (19.43±2.84) μg/L] increased significantly( t = - 2. 8085, P ＜0.05) . Compared with sham after injury [ ( 11.12±1.27 ) ng], adipose leptin levels of I60-R30[ (4.46±2.63) ng]and I60-R90[ (3, 45±2.77) ng] decreased significantly( t = 2. 2804 and 2. 5170, respectively, both P＜0.05), while that of I60-R360[(17.54±1.87)ng] increased significantly( t = -2. 8401, P＜0.05).CONCLUSION: Leptin has a time-dependent response to acute inflammatory stimuli and participates in the energy metabolic disorders of acute inflammation.关键词
再灌注损伤,肠/瘦素/放射免疫检测/脂肪组织分类
医药卫生引用本文复制引用
林季,颜光涛,王录焕,郝秀华,张凯,薛辉..肠缺血再灌注损伤对血清及脂肪组织Leptin水平的影响[J].中国临床康复,2004,8(29):6519-6521,3.基金项目
国家自然科学基金(39970717)资助项目 （39970717）
