动物学研究2005，Vol.26Issue(6)：652-656,5.
牙鲆CA/GT微卫星标记的筛选
Isolation of CA/GT Microsatellites from the Paralichthys olivaceus Genome
摘要
Abstract
A library rich in CA/GT microsatellites was constructed from the Paralichthys olivaceus genome by combining biotin capture method and radioactive labeling hybridization. Five hundred and twenty six positive clones were obtained through twice screens. Sequencing confirmed 133 microsatellite loci (number of repeats≥5) in 119 positive clones. Of these microsatellites, two (1.5%) had compound repeat motifs, 63 (47.37%) had perfect motifs and 68 (51.13%) had imperfect motifs. Primer pairs were designed in the flanking regions of 22 microsatelites and subjected to PCR amplification. In 8 artificial gynogenesis families, four pairs failed to amplification, one pair was monomorphic, and the rest were polymorphic with an average of 5.2 alleles per locus. Heterozygosities ranged between 0.375 and 0.846, PIC ranged between 0.305 and 0.823. The results suggested that most of the microsatellites we isolated were qualified to be applied to the population genetic studies of P. olivaceus.关键词
牙鲆/微卫星标记/生物素选择杂交法/同位素标记杂交法Key words
Paralichthys olivaceus/Microsatellite/Biotin capture/Radioactive labeling分类
生物科学引用本文复制引用
常玉梅,孙效文,李绍武,赵莹莹,朱晓琛,刘海金..牙鲆CA/GT微卫星标记的筛选[J].动物学研究,2005,26(6):652-656,5.基金项目
国家 "973" 计划(2004CB117405) （2004CB117405）
