首页|期刊导航|中国农业科学(英文版)|Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment
中国农业科学(英文版)2007,Vol.6Issue(6):652-656,5.
Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment
Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment
摘要
Abstract
In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 (White), which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.关键词
maize/inbred line/Barstar gene/particle bombardment/transgenic plant/molecular detectionKey words
maize/inbred line/Barstar gene/particle bombardment/transgenic plant/molecular detection分类
农业科技引用本文复制引用
SUN Qing-quan,ZHANG Ying,RONG Ting-zhao,DONG Shu-ting,ZUO Zhen-peng..Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment[J].中国农业科学(英文版),2007,6(6):652-656,5.基金项目
This study was funded by the National Doctoral Fund of China,Hortation Fund for Excellent Youth Scientist of Shandong Province, China(2005BS06010),National Program on Key Basic Research Projects of China (973 Program,2006CB101700),Choiceness Seed Industrialization Item of Shandong Province,China (Lu TA 2004 113),Science and Technology Innovation Fund of Shandong Agricultural University,China. (2005BS06010)