中国临床康复2004，Vol.8Issue(29)：6542-6544,3.
超氧化物歧化酶模型配合物对大鼠骨骼肌缺血再灌注损伤的保护作用
Effects of superoxide dismutase model coordination compound on the skeletal muscle ischemia-reperfusion injury of rats
摘要
Abstract
BACKGROUND: Treatment of skeletal muscle ischemia-reperfusion injury has been an important and difficult task for orthopedicians.OBJECTIVE: To explore the protective function and mechanism of superoxide dismutase(SOD) model coordination compounds MSODa in skeletal muscle ischemia-reperfusion injury, and thus provides a theoretical basis for the rehabilitation of skeletal muscle injury.DESIGN: Randomized controlled studySETTING and MATERIALS: The experiment was completed in the Second Hospital affiliated to Harbin Medical University. Ninety-six clean Wistar rats, with the mean mass of(200±20) g, were provided by the experimental animal center of the Second Hospital affiliated to Harbin Medical University.INTERVENTIONS: Rat hind limb ischemia-reperfusional models were successfully established. Ninety-six rats were randomly divided into four groups: normal control group(group Ⅰ, n = 6), ischemia-repeffusion group (group Ⅱ, n = 30), normal saline group (groupⅢ, n = 30), and MSODa group(group Ⅳ, n = 30) . The pathological changes were assessed 4 hours after ischemia, and 1 hour, 2 hours, 4 hours, 8 hours, and 12 hours after reperfusion in group Ⅱ, groupⅢ, and groupⅣ.MAIN OUTCOME MEASURES: Malondialdehyde(MDA) in plasma,myeloperoxidase(MPO) in skeletal muscle, CD11b/CD18 on leukocytes,and intercellular adhesion molecule-l(ICAM-1) in skeletal muscular yessels, as well as the pathological changes of each group were measured.RESULTS: The expression of MDA, MPO, CD11b/CD18, and ICAM-1 increased significantly in group Ⅱ compared with group Ⅰ. Skeletal muscle injury aggravated along with the extension of reperfusion time, but such changes were significantly improved in group Ⅳ.CONCLUSION: MSODa can prevent skeletal muscle ischemia-repeffusion injury by inhibiting the production of free radicals and the expression of adhesion molecules.关键词
再灌注损伤/肌,骨骼/超氧化物歧化酶分类
医药卫生引用本文复制引用
王新涛,吕松岑,韩竹,关德宏,常曼丽..超氧化物歧化酶模型配合物对大鼠骨骼肌缺血再灌注损伤的保护作用[J].中国临床康复,2004,8(29):6542-6544,3.基金项目
黑龙江省自然基金资助(D9827) （D9827）
