中华医学杂志（英文版）2001，Vol.114Issue(6)：654-657,4.
套式PCR检测蚊体内间日疟原虫子孢子
Nested polymerase chain reaction in detection of Plasmodium vivax sporozoites in mosquitoes
李凤舞 1牛春 1叶炳辉2
作者信息
- 1. 首都医科大学细胞生物系北京 100054
- 2. 南京医科大学寄生虫教研室南京 210029
- 折叠
摘要
Abstract
Objective To detect malaria DNA in mosquitoes.
Methods A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used.
Results In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121?bp band was seen with DNA templates from other malaria parasites or negative mosquitoes.
Conclusion Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.关键词
套式PCR检测 间日疟原虫 DNA 特异性Key words
Plasmodium vivax/mosquito/nested polymerase chain reaction/detection
分类
医药卫生引用本文复制引用
李凤舞,牛春,叶炳辉..套式PCR检测蚊体内间日疟原虫子孢子[J].中华医学杂志（英文版）,2001,114(6):654-657,4.
