首页|期刊导航|中国农业科学（英文版）|Establishment of Ecotilling for Discovery of DNA Polymorphisms in Brassica rapa Natural Population
中国农业科学（英文版）2005，Vol.4Issue(9)：654-659,6.
Establishment of Ecotilling for Discovery of DNA Polymorphisms in Brassica rapa Natural Population
Establishment of Ecotilling for Discovery of DNA Polymorphisms in Brassica rapa Natural Population
摘要
Abstract
Ecotilling is a new approach based on enzyme-mediated heteroduplex cleavage to discover DNA polymorphisms in natural population. We used mung bean nuclease(MBN) instead of routinely used CELI to cleave single base pair mismatches in heteroduplex DNA templates. Nested set of primers were designed to amplify targeted region to avoid the influence of the variation in quality and quantity of the genomic DNA. To reduce the costs in fluorescently labeled primers, we added M13 adapter to 5'end of gene specific primers to make IRD dye labeled M13 forward and reverse primers possibly universal for different genes. A Brassica rapa ZIP gene homologue was subjected to the analysis to practise the feasibility of the method in polymorphisms detection. Our experiment showed this method is efficient in discovering DNA polymorphisms in Brassica rapa natural population.关键词
Ecotilling/MBN/DNA polymorphismKey words
Ecotilling/MBN/DNA polymorphism分类
农业科技引用本文复制引用
WU Jian,SUN Ri-fei,ZHANG Yan-guo,WANG Xiao-wu..Establishment of Ecotilling for Discovery of DNA Polymorphisms in Brassica rapa Natural Population[J].中国农业科学（英文版）,2005,4(9):654-659,6.基金项目
The work was funded by The Opening Lab of Vegetable Genetics and Physiology of Ministry of Agriculture and Sino-Dutch Joint Lab of Horticultural Genomics Technology, P.R.China. （）
