南京林业大学学报(自然科学版)2004,Vol.28Issue(z1):65-70,6.
利用RAPD标记研究桂林桂花品种间的亲缘关系
Identifying Osmanthus fragrans Cultivars in Guilin City and Evaluating Their Genetic Relationships by RAPD Markers
摘要
Abstract
Random Amplified Polymorphic DNA(RAPD) markers were applied to analyze 23Osmanthus fragrans cultivars in Guilin City. Among the total 193 fragments generated by 20 selected primers (among 100 primers), 114 appeared to be polymorphic(59.1%). Cluster analysis based on the RAPD results was performed and dendrogram was constructed. These cultivars tested by RAPD were divided into 4 cultivar groups. The result was consistent with that from traditional taxonomy analysis. The RAPD study illustrated that it is possible to analyze intra-cultivar variation of O. fragrans cultivars on the basis of genotype rather than phenotype and therefore, offered significant evidences in solving taxonomic problem of O. fragrans cultivars in Guilin City.关键词
RAPD/桂林/桂花/亲缘关系Key words
RAPD/Guilin/Osmanthus fragrans Lour./Genetic relationship分类
农业科技引用本文复制引用
伊艳杰,黄莹,尚富德..利用RAPD标记研究桂林桂花品种间的亲缘关系[J].南京林业大学学报(自然科学版),2004,28(z1):65-70,6.基金项目
河南省自然科学基金资助项目(0311031400) (0311031400)