A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus PlantsOA
A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants
We report here a seed transformation of sonication-assisted,no-tissue culture to rapidly produce transgenic Brassica napus plants.This method comprises the steps of treating seeds by ultrasonic wave,inoculating Agrobacterium …查看全部>>
We report here a seed transformation of sonication-assisted,no-tissue culture to rapidly produce transgenic Brassica napus plants.This method comprises the steps of treating seeds by ultrasonic wave,inoculating Agrobacterium tumefaciens with a recombinant ChlFN-a gene and germinating directly of treatment seed on wet filter papers.The obtained transformants were verified by GUS histochemical assay and nested PCR amplification.It suggests that seed transforma…查看全部>>
SONG Li;ZHAO De-gang;WU Yong-jun;TIAN Xiao-e
Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,P.R.ChinaKey Laboratory of Green Pesticide and Agricultural Biological Engineering,Ministry of Education/Guizhou University,Guiyang 550025,P.R.ChinaGuizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,P.R.ChinaKey Laboratory of Green Pesticide and Agricultural Biological Engineering,Ministry of Education/Guizhou University,Guiyang 550025,P.R.China
农业科学
Brassica napusseed transformationsonication
Brassica napusseed transformationsonication
《中国农业科学（英文版）》 2009 (6)
658-663,6
This work was supported by research grants from the National Key Technology Research and Development Program of China (2007BAD59B06),the International Science and Technology Cooperation Program of China(2007DFA31260),and the Science Foundation for the Excellent Youth Scholars of Guizhou Province of China to Zhao Degang(20030312).
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