作物学报2003，Vol.29Issue(5)：664-669,6.
大豆品种的再生性能及对EHA 101农杆菌的敏感性
Regeneration Study of Soybean Cultivars and Their Susceptibility to Agrobacterium tumifaciens EHA 101
摘要
Abstract
The soybean transformation procedure included the Agrobacterium-cotyledonary node system and the bar gene as the selectable marker coupled with glufosinate as a selective agent. Cotyledonary nodes from 5 to 6 days germinated soybean seeds were used as explants. The explants, were wound by slicing 5 to 6 times, inoculated with Agrobacterium tumefaciens EHA 101 with pPTN140, then followed 3 days co-cultivation, washed the explants by wash medium which contain antibiotics, put explants onto shoot initiation medium with 5 mg/L glufosinate for selection. Regeneration rate of different soybean cultivars was counted 2 weeks later, and their susceptibility to Agrobacterium tumefaciens was investigated 4 weeks later by GUS assay. According to our experiments, Heinong 35, Zhongzuo 975(Zhonghuang 13), Hefeng 35, Zhongzuo962 are better than Thorne for regeneration, William 82, PI 361066, Heinong 35 and Zhongzuo 975 were better than Thorne for transformation.关键词
大豆/再生/对农杆菌的敏感性Key words
Soybean/Regeneration/Susceptibility to Agrobacterium分类
农业科技引用本文复制引用
王岚,T Clemente,王连铮,辛世文,黄其满..大豆品种的再生性能及对EHA 101农杆菌的敏感性[J].作物学报,2003,29(5):664-669,6.基金项目
This research was supported by Nobel Foundation in USA from 1999-2000 and 948 project from MOA,PRC. （）
