中国农业科学（英文版）2009，Vol.8Issue(6)：664-670,7.
Cloning and Expression of Key Enzyme Gene GalAT in Ramie Pectin Biosynthesis
Cloning and Expression of Key Enzyme Gene GalAT in Ramie Pectin Biosynthesis
摘要
Abstract
To isolate the eDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea (L.)Gaud],and thus to understand the expression of GalAT gene in different tissues of ramie,degenerate primer was designed according to GalAT conserved sequence in other species reported,and the eDNA sequence of GalAT gene from ramie variety Zhongzhu 1 was cloned by RT-PCR method based on the degenerate primer.The eDNA revealed a 986-bp in length which encoded 328 amino acids.The eDNA sequence and putative amino acid sequence of GalAT shared high identity with previously reported Arabidopsis thaliana GAUT4 (GalAT) as 77 and 83%,respectively.Molecular evolution analysis showed that the putative amino acid sequence and Arabidopsis thaliana GAUT4 gathered to a same group.Real-time quantitative PCR analysis showed that GalAT mRNA accumulated most abundantly in root,and GalAT transcripts in all kinds of ramie tissues in turn revealed as follows:root ＞ leaf＞ bast ＞ or ≈ xylem.关键词
clone/GalAT/pectin/ramie/real-time quantitative PCR analysisKey words
clone/GalAT/pectin/ramie/real-time quantitative PCR analysis分类
农业科学引用本文复制引用
LIU Jian-xin,YU Chun-ming,TANG Shou-wei,ZHU Ai-guo,WANG Yan-zhou,ZHU Si-yuan,MA Xiong-feng,XIONG He-ping..Cloning and Expression of Key Enzyme Gene GalAT in Ramie Pectin Biosynthesis[J].中国农业科学（英文版）,2009,8(6):664-670,7.基金项目
This work was supported by the National 863 Program of China (2001AA241211). （2001AA241211）
