中华医学杂志（英文版）2004，Vol.117Issue(5)：668-672,5.
Endogenous erythroid colony assay in patients with polycythemia vera and its clinical significance
Endogenous erythroid colony assay in patients with polycythemia vera and its clinical significance
白洁 1邵宗鸿 1刘鸿 1施均 1何广胜 1曹燕然 1崔振珠 1吴玉红 1孙娟 1田征 1贾海蓉 1钱林生 1杨天楹 1杨崇礼1
作者信息
- 1. Department of Clinical Hematology, Blood Diseases Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300020, China
- 折叠
摘要
Abstract
Background Polycythemia vera (PV) is a malignant disorder of hemaopoietic stem cells which is characterized by clonal hyperproliferation and a low rate of apoptosis. This study was to assess endogenous erythroid colony (EEC) formation in the bone marrow of PV patients and determine its clinical significance.Methods The bone marrow mononuclear cells of 26 patients with PV, 2 patients with secondary erythrocytosis (SE), and 19 normal controls were cultured by Marsh's method for EEC evaluation, and the clinical significance was evaluated.Results EECs appeared in 25 patients with PV but not in 2 patients with SE and 19 normal controls. The number of EECs and the EEC ratio [EEC/erythropoietin (EPO)-dependent colony forming unit-erythroid (CFU-E)] in PV patients positively correlated with hemoglobin (Hb) levels. Their EEC number did not correlate with white blood cell (WBC) counts, platelet (PLT) counts, or leukocyte alkaline phosphatase (LAP) scores. Their EEC did not correlate with serum EPO levels. Fifteen patients with PV were treated with hydroxyurea (Hu) and/or interferon-alpha (IFN-α). Their EEC ratio before treatment positively correlated with the treatment time required for complete remission (CR) and negatively correlated with the time before relapse. The EEC numbers of 7 PV patients treated with Hu/IFN-α decreased after the blood cell counts dropped to normal levels. There was a positive correlation between the EEC ratio and the incidence of attacks of vascular thrombosis in PV patients. The numbers of apoptosised bone marrow mononuclear cells in PV patients were lower than those in normal controls. The EEC numbers of PV patients negatively correlated with the rate of apoptosis of bone marrow mononuclear cells.Conclusions EEC formation is characteristic in PV patients. EEC number in PV patients positively correlates with Hb levels, the time required for CR, and the incidence of attacks of vascular thrombosis. EEC number negatively correlates with the time before relapse. Bone marrow suppressive treatment might decrease EEC number. Thus, EEC number is a sensitive and specific parameter reflecting the abnormal hematopoietic clone burden induced by polycythemia vera. EEC number is an important diagnostic parameter for PV patients.关键词
polycythemia vera/endogenous erythroid colony/clinical significanceKey words
polycythemia vera/endogenous erythroid colony/clinical significance分类
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白洁,邵宗鸿,刘鸿,施均,何广胜,曹燕然,崔振珠,吴玉红,孙娟,田征,贾海蓉,钱林生,杨天楹,杨崇礼..Endogenous erythroid colony assay in patients with polycythemia vera and its clinical significance[J].中华医学杂志（英文版）,2004,117(5):668-672,5.
