湖南大学学报（自然科学版）2009，Vol.36Issue(5)：67-70,4.
基于壳聚糖纳米颗粒的基因枪法转化洋葱细胞研究
Chitosan Nanoparticle as Gene Vehicle Mediated by Particle Bombardment in Onion Cell Transformation
摘要
Abstract
Chitosan nanoparticles were synthesized with cocervation process.The particles were characterized with transmission electron microscope and Zeta-Sizer measurement.Chitosan nanoparticles were shown to be spherical structure distributed evenly, the average sizes of the particles were about 50 nm in diameter, and the particles were 11.1 mv charge.Plasmid DNA was absorbed to chitosan nanoparticles by electrostafic forces.Agarose gel electrophoresis showed that the pEGAD plasmid DNA was effectively absorbed to chitosan nanoparticles.Furthermore, the complexes could protect the absorbed DNA from DNase I damage.Chitosan nanoparticles-DNA mixed was transformed into the onion cell via particle bombardment, and onion cells were observed under invert-fluorescence-microscope.The results showed that transformed onions with GFP gene were obtained by particle bombardment successfully.Analysis showed that the reporter GFP gene was expressed with high efficiency (8 %).关键词
纳米颗粒/基因枪/转化/洋葱细胞Key words
nanoparticle/particle bombardment/transformation/onion cell分类
化学化工引用本文复制引用
王凤华,刘俊,唐冬英,薛昌刚,童春义,吴宪远,刘选明..基于壳聚糖纳米颗粒的基因枪法转化洋葱细胞研究[J].湖南大学学报（自然科学版）,2009,36(5):67-70,4.基金项目
高校国家"985工程"科技创新平台资助项目(200501) （200501）
