植物研究2008，Vol.28Issue(1)：67-72,6.
飞机草花蕾中Beclin1基因的克隆与表达
Cloning and Characterization of Beclin1 -like Gene in Eupatorium odoratum L. Buds
祁长青 1刘英 1王俐 1李思滨 1王敏 1姜洋 1祖元刚1
作者信息
- 1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨,150040
- 折叠
摘要
Abstract
Beclin1 -like gene was cloned from three different growth periods flower buds of Eupatorium odoratum by RT-PCR method in this study. The homology between Beclin1 -like gene (about 700 bp)and Beclin1 gene in tobacco leaves is 95 %; Northern blotting result showed that the expression of Beclin 1 -like gene in the middle stage was higher than those in initial stage and dernier stage; the result of DNA ladder showed Programmed Cell Death (PCD) happened in the developing process of flower buds.All the results proved that PCD certainly happened with the tapetum degenerating and the pollen coming into being, which indicated the relationship between the aggressive process of E. odoratum and PCD in the process of the reproduction.关键词
飞机草/花蕾/Beclin1基因/细胞程序化死亡/绒毡层Key words
Eupatorium odoratum/ buds/ Beclin1 gene/ Programmed Cell Death (PCD)/ tapetum分类
生物科学引用本文复制引用
祁长青,刘英,王俐,李思滨,王敏,姜洋,祖元刚..飞机草花蕾中Beclin1基因的克隆与表达[J].植物研究,2008,28(1):67-72,6.
