南京医科大学学报(英文版)2004，Vol.18Issue(2)：67-77,11.
Analysis of the Relationship between Genome Diversity and Adult Survive Rate of Botryllus Schlosseri by AFLP
Analysis of the Relationship between Genome Diversity and Adult Survive Rate of Botryllus Schlosseri by AFLP
FENG Xiao-rong 1ZHU Jian-zhong 1DENG Feng-jiao 2Jacob Douek 3Baruch Rinkevich3
作者信息
- 1. Department of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA 170033, USA
- 2. Institute of Life Sciences, laboratory of Animal Development and Genetics, Wuhan University, Wuhan 430072, P.R.China
- 3. Center for Immunology and Development Biology, National Institute of Oceanography, Tel-Shiknona, POB 8030, Haifa 31080, Israel
- 折叠
摘要
Abstract
Objective: The self-cross colonial prochordate, Botryllus schlosseri ( B. schlosseri) occupy a key phylogenetic position in the evolution of vertebrates. To clarify the relationship of genome diversity and survive rate, five generations of B.schlosseri was investigated by amplified fragment length polymorphism (AFLP). Methods:AFLP markersare extremely sensitive to even smell sequence variation, using PCR and high-resolution electrophoresis to examine restriction fragments. Results: AFLP polymorphism was high in the parent and lower in its F1, F2, F3 and F4. Each primer combination generated from 80 to more than 120 bands, of which average 25.85% poiymorphic loci in parent, 15.79% polymorphic among F1, 9.16% and 5.58% in F2,F3. The AFLP markers were transmitted from F1 to F2, F3 and F4 and inherited, segregated in expected Mendelian ratio. However, some of the markers were lost in F2, F3 and F4 while it disappeared in their mother. In addition, gene mutation-new loci and lost loci among F1, F2, F3 and F4 were observed. These special fragments were cloned and sequenced. Then, the genomic DNA was analyzed by Southem hybridization with the probes from these specific fragments and the mechanism of gene mutation was clarified. Conclusion:The se results suggest that there are high frequency of polymorphic loci and mutation in genome of B.schlosseri. Gene deletion or iow diversity may be the reason for high rate of death of the offspring of inbred laboratory-reared strains.关键词
Botryllus schlosseri/genome diversity/mutation/amplified fragment length polymorphism/polymorphism/survive rateKey words
Botryllus schlosseri/genome diversity/mutation/amplified fragment length polymorphism/polymorphism/survive rate分类
生物科学引用本文复制引用
FENG Xiao-rong,ZHU Jian-zhong,DENG Feng-jiao,Jacob Douek,Baruch Rinkevich..Analysis of the Relationship between Genome Diversity and Adult Survive Rate of Botryllus Schlosseri by AFLP[J].南京医科大学学报(英文版),2004,18(2):67-77,11.
