中风与神经疾病杂志2001，Vol.18Issue(1)：6-8,3.
多巴胺抑制PC12细胞增殖和诱导凋亡作用的研究
The study of proliferation inhibition and apoptosis induced by dopamine on PC12 cell
李立宏 1高国栋 1张宝国 1王学廉 1李向忠1
作者信息
摘要
Abstract
Objective To study the effect of dopamine in inhibition of proliferation and induction of apoptosis in PC12 cell, and explore the mechanism of dopaminergic neuronal death in substantia nigra with Parkinson's disease (PD). Methods Based on the immunohistochemical technology, the study of proliferation inhibition and apoptosis induced by dopamine in PC 12 cell was examined by flow cytometry, electron microscope and electrochromatography. Results Moderate concentration of dopamine could inhibit significantly the growth of PC 12 cells and induce apoptosis. DA could inhibit the proliferation within 12 hours and the typical apoptosis peak was not observed by flow cytometry, but exhibit a obvious inhibition on S phase of the cell cycle and the Bcl-2 expression could be found and the special alteration of mitochondria and endoplasmic reticulum could be found in electromicroscope. DA could induce apoptosis on PC12 cell beyond 24 hours, and the special apoptosis peak could be found by flow cytometry, the typical DNA“ladder” fragments by electrochromatography and specific ultrastructure changed in electromicroscope such as pyknosis could be found in the same time. Conclusions Dopamine can inhibit proliferation and induce the apoptosis on PC 12. And apoptosis may be involved the pathogenesis of PD.关键词
凋亡/增殖/PC12细胞/帕金森病分类
医药卫生引用本文复制引用
李立宏,高国栋,张宝国,王学廉,李向忠..多巴胺抑制PC12细胞增殖和诱导凋亡作用的研究[J].中风与神经疾病杂志,2001,18(1):6-8,3.
