中国人兽共患病杂志2000，Vol.16Issue(6)：6-8,3.
日本血吸虫中国大陆株重组脂肪酸结合蛋白(sj-FABPc)的特性和抗原表位的分析
STUDIES ON THE CHARACTERISTIC AND ANTIGENIC EPITOPE OF A ECOMBINANT PROTEIN OF FATTY ACID-BINDING PROTEIN (FABPc)FROM SCHISTOSOMA JAPONICUM
摘要
Abstract
im To analyse the characteristic and antigen epitope of recombinant antigen Sj-FABPc, Methods The cloned gene of FABP was amplified by PCR from a Schistosoma japonicum adult worm cDNA library and subcloned into plasmid pGEM-T. The sequence was analysed by GOLDKEY DNA and Protein Analytical Program and Swiss-Model Protein Modelling server in Internet. Results The Tesult indicated that the cloned gene was Sj-FABPc. Three possible antigenic epitopes and three sites of binding fatty acid,of recombinant antigen of Sj-FABPc ,were predicted by computer. Conclusions The antigen epitopes of Sj-FABPc and the main sites of binding fatty acid may share same peptide.关键词
日本血吸虫/脂肪酸结合蛋白(FABP)/重组抗原/序列分析/同源性/抗原表位Key words
Schistosoma japonicum/Fatty acid binding protein (FABP)/Recombinant antigen/Sequence analysis/antigenic epitope分类
医药卫生引用本文复制引用
赵巍,关海玮,苏川,胡雪梅,沈蕾,陈淑贞,张兆松..日本血吸虫中国大陆株重组脂肪酸结合蛋白(sj-FABPc)的特性和抗原表位的分析[J].中国人兽共患病杂志,2000,16(6):6-8,3.基金项目
本研究课题获江苏省重点实验室开放基金资助作者单位： （）
