中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(12)：682-685,4.
实时荧光PCR法检测肺癌细胞系中吉西他滨耐药相关基因的表达
Detection of gemcitabine-resistant genes expression in lung cancer cell lines using real-time PCR
摘要
Abstract
Objective: To explore the molecular mechanism of gemcitabine-resistance, the relative mRNA expression of five genes related to gemcitabine-resistance was detected in six lung cancer cell lines. Methods: The total RNA was extracted from six lung cancer cell lines GLC-82, NCl-H460, A549, 95-C, 95-D and QG56. Then the cDNA was amplified by real-time quantitative PCR method to quantify the gene expression of RRM1, PTEN, ERCC1, dCK and CDA. The cytotoxicity of gem-citabine to cell lines was tested by MTT method. Results: Among the detected six lung cancer cell lines, the mRNA level of RRM1, PTEN and ERCC1 in lung squamous cell line QG56 was highest, and the IC50 of gemcitabine to QG56 cell line was also highest. Conclusion: The mRNA expression of RRM1, PTEN and ERCC1 was correlated, and the high expression of RRM1 was related to gemcitabine resistance of lung cancer.关键词
gemcitabine-resistant/real-time PCR/gene expression/lung cancerKey words
gemcitabine-resistant/real-time PCR/gene expression/lung cancer分类
临床医学引用本文复制引用
Li Lin ,Xiaoqing Liu ,Yalan Rao ,Weixia Wang ,Shengqi Wang ,Santai Song..实时荧光PCR法检测肺癌细胞系中吉西他滨耐药相关基因的表达[J].中德临床肿瘤学杂志（英文版）,2008,7(12):682-685,4.基金项目
a grant from the Capital Medical Developing Foundation of China (No. 03028). （No. 03028）
