水生生物学报2009，Vol.33Issue(4)：682-689,8.DOI:10.3724/SP.J.0000.2009.40682
饲料中不同维生素C含量对长吻鮠的影响
EFFECTS OF DIFFERENT DIETARY VITAMIN C LEVELS ON CHINESE LONGSNOUT CATFISH (LEIOCASSIS LONGIROSTRIS GUNTHER)
摘要
Abstract
Vitamin C is an essential micro-nutrient for most fish and plays an important role in immunity and physiology. The deficiency of dietary vitamin C can result in the damage on fish. The study on the deficiency symptom and the sensitive indicator of vitamin C could provide useful information for fish feed industry. This experiment was conducted to investigate the effects of dietary Vitamin C levels on growth performance, immune and physiological responses in Chinese longsnout catfish (Leiocassis longirostris Gunther) , which is an high value aquaculture fish in China. Three practical diets containing 38,364 and 630mg/kg vitamin C were fed to Chinese longsnout catfish (23. 19 ± 0. 58g) for 8 months. Vitamin C was used in the form of L-ascorbyl-2-polyphosphate. The results indicated that the Chinese longsnout catfish in 38mg/kg vitamin C groups showed typical vitamin C deficiency symptom: skin color turning darker, spinal deformities and scoliosis, erosion of fins, anaemia. SGR and the serum lysozyme activities decreased significantly (p ＜ 0. 05), the hepatic SOD activities and MDA contents increased significantly (p ＜ 0. 05). There was no significant difference in serum cortisol concentrations (p ＞ 0. 05). The inductive HSP70 in liver was not detected by western-blotting in all groups. In conclusion, serum cortisol and hepatic HSP70 were not the sensitive indicators for vitamin C deficiency symptom, but the serum lysozyme, hepatic SOD activity, hepatic MDA content and the haematological index (red blood cell number and hemoglobin content) were the more reliable makers to indicate the physiological status in Chinese longsnout catfish.关键词
维生素C/维生素C缺乏症/长吻鮠Key words
Vitamin C/Vitamin C deficiency symptom/Chinese longsnout catfish分类
农业科技引用本文复制引用
刘海燕,雷武,朱晓鸣,杨云霞,韩冬,薛敏,解绶启..饲料中不同维生素C含量对长吻鮠的影响[J].水生生物学报,2009,33(4):682-689,8.基金项目
湖北省水产重大专项(2006AA203A01) （2006AA203A01）
十一五国家科技支撑计划(2006BAD03B01) （2006BAD03B01）
淡水生态与生物技术国家重点实验室开放基金(2009FB09)资助 （2009FB09）
