中国林学（英文版）2006，Vol.8Issue(4)：6-9,4.
Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L.
Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L.
摘要
Abstract
Agrobacterium-mediated genetic transformation of Sophorajaponica was standardized using the Agrobacterium tumefaciens strain LBA4404 that harbored the binary vector pBI121 containing genes for β-glucuronidase (GUS) and neomycin phosphotransterase (npt Ⅱ). S. japonica transformants were selected by the ability of the leaf explants to produce kanamycin-resistant calli that regenerated into kanamycin-resistant plantlets. Successful transformation was confirmed by histochemical assay for GUS activity, PCR analysis and Southern blot. The period of nearly two months was required for the regeneration of transgenic plantlets from the explants. The transformed plants resembled their parents in morphology.关键词
β-glucuronidase (GUS), Sophora japonica, Agrobacterium tumefaciens, neomycin phosphotransterase (npt Ⅱ),transformationKey words
β-glucuronidase (GUS), Sophora japonica, Agrobacterium tumefaciens, neomycin phosphotransterase (npt Ⅱ),transformation分类
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Zhang Xiao-ying,Wang Hua-fang,Yin Wei-lun,Zhu Zhen..Agrobacterium tumefaciens-mediated GUS gene transfer to Sophora japonica L.[J].中国林学（英文版）,2006,8(4):6-9,4.基金项目
The study was supported by High-Tech Research and Development Program of China (Grant No. 2001AA244041). （Grant No. 2001AA244041）
