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Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria

哈尔滨工业大学学报（英文版）2007，Vol.14Issue(5)：691-694,4.

Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria

作者信息

1. Chemical Engineering,Shanghai University of Engineering and Sciences,Shanghai 201620,China;Forestry School,Northeast Forestry University,Harbin 150040,China;Municipal and Environmental Engineering School,Harbin Institute of Technology,Harbin 150090,China;Chemical Engineering,Shanghai University of Engineering and Sciences,Shanghai 201620,China;Forestry School,Northeast Forestry University,Harbin 150040,China;Municipal and Environmental Engineering School,Harbin Institute of Technology,Harbin 150090,China
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摘要

Abstract

A method based on PCR amplification of the 16S rRNA gene (rDNA) -23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262, 398, 638, 437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261, 579 and 485 bp. Of the 3 species of the novel group hydrogenproducing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated With any Clostridium sp. Studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.

关键词

Biohydrogen production/Hydrogen-producing Anaerobes/ISR of 16S-23S rDNA/Length polymorphisms

Key words

Biohydrogen production/Hydrogen-producing Anaerobes/ISR of 16S-23S rDNA/Length polymorphisms

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..Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria[J].哈尔滨工业大学学报（英文版）,2007,14(5):691-694,4.

基金项目

Sponsored by Prigram of shanghai Education Committee(Grant No.07ZZ156) （Grant No.07ZZ156）

Key Subject Construction of Shanghai Education Committee(Grant No.P1402) （Grant No.P1402）

the National Natural Science Fund of China(GrantNo.30470054). （GrantNo.30470054）

哈尔滨工业大学学报（英文版）

ISSN：1005-9113

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