水生生物学报2009，Vol.33Issue(4)：696-701,6.DOI:10.3724/SP.J.0000.2009.40696
溴化1-己基-3-甲基咪唑对斜生栅藻谷胱甘肽及其代谢酶的影响
RESPONSES OF GLUTATHIONE CYCLE ENZYMES AND GLUTATHIONE METABOLISM TO 1-HEXYL-3-METHYLIMIDAZOLIUM STRESS IN SCENEDESMUS OBLIQUUS
摘要
Abstract
The effects of ionic liquid 1-hexyl-3-methylimidazolium ([C6 mim] Br), with the concentrations of 0, 1,5, 10, 15 and 20 mg/L and exposed for the 24, 48,72 and 96h respectively, on glutathione (GSH) and the enzymes in glutathione metabolism of Scenedesmus obliquus such as glutathione peroxidase (GPX) , glutathione S-transferases (GST) and glutathione reductase (GR) were studied in the present study. The results indicated that the GSH contents decreased with the concentration of [C6 mim] Br increasing at the 24, 48 and 72h, but it did not change when [C6 mim] Br = 1 mg/L. The GSH contents were similar to or lower than that of the control level at the 96h. The activities of GPX and GST increased markedly before 72h except for the activity of GST at the highest concentration (20 mg/L), but they both decreased to the control level at the 96h. The activities of GR increased at the concentration of 1 mg/L and then decreased with the concentration increasing at the 24h, increased to the control level at the 48h, and then reached its peak at the 72h (when [C6 mim] Br≥10 mg/L the GR activities exceeded the control level), but at the 96h, it decreased markedly even weaker than the control level. GR was the enzyme that limited the reaction velocity in the system of glutathione cycle and the GST was the most active and sensitive enzyme. It could be used as sensitive biomarkers under the stress induced by [C6 mim] Br. [C6 mim] Br induced oxidative stress of S. obliquus at the concentration of 1 mg/L and had environmental toxicity.关键词
离子液体/斜生栅藻/还原型谷胱甘肽/谷胱甘肽转硫酶/谷胱甘肽过氧化物酶/谷胱甘肽还原酶Key words
Ionic liquid/Scenedesmus obliquus/Glutathione/Glutathione S-transferases/Glutathione peroxidase/Glutathione reductase分类
资源环境引用本文复制引用
马剑敏,蔡林林,胡灵卫,靳同霞,李效宇,王键吉..溴化1-己基-3-甲基咪唑对斜生栅藻谷胱甘肽及其代谢酶的影响[J].水生生物学报,2009,33(4):696-701,6.基金项目
国家自然科学基金(20273019,20573034) （20273019,20573034）
河南省高校杰出人才计划(2006KYCX021) （2006KYCX021）
河南省科技攻关计划(0624440039)资助 （0624440039）
