中国林学（英文版）2006，Vol.8Issue(4)：69-73,5.
Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia 'Idaho'
Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia 'Idaho'
摘要
Abstract
Robinia pseudoacacia 'Idaho' (Robinia × ambigua 'Idahoensis', R. pseudoacacia × R. viscosa) modified by a mtl-D gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by previous investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory.For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.关键词
Robinia pseudoacacia 'Idaho'/rooting/acclimatization/micropropagation/in vitroKey words
Robinia pseudoacacia 'Idaho'/rooting/acclimatization/micropropagation/in vitro分类
农业科技引用本文复制引用
Xie Jun-wen,Li Min,Chen Shou-yi,Sun De-you,Wang Hua-fang..Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia 'Idaho'[J].中国林学（英文版）,2006,8(4):69-73,5.基金项目
This study was supported by the National High Technology and Development Program of China (Grant No.2004AA244040) and the National Research and Development Program of China (JY03-B-29). （Grant No.2004AA244040）
