扬州大学学报（农业与生命科学版）2009，Vol.30Issue(2)：69-75,7.
辣椒疫霉EST-SSRs标记的发掘
Mining simple sequence repeats markers in expressed sequence tags of Phytophthora capsici
摘要
Abstract
Simple sequence repeats (SSRs) were identified through bioinformatic mining of 55 848 publicly available expressed sequence tags (ESTs) of Phytophthora capsici in dbEST GenBank. The 331 SSRs were identified in 328 unique ESTs with each containing one or two SSRs and an average density of one SSR per 23. 7 kb of EST sequence screened. The trinucleotide motifs were the most abundant type of SSRs (59.5%) followed by dinucleotide (39.3%) and tetranucleotide repeats (1.2%). Forty EST-SSR primer pairs were designed and 27 primer pairs amplified characteristic SSR fragments of expected sizes when tested with genomic DNA of five P. capsici strains. Eighteen primer pairs amplified polymorphic microsatellite markers and 25 polymorphic markers were characterized for the five test strains. The dendrogram of the five strains was generated using the UPGMA clustering methods and the strains were clustered into three groups according to the 25 polymorphic EST-SSR markers. This is the first report of developing SSR markers of P. capsici that may contrib-ute to more comprehensive analyses of genetic diversity of this important pathogen.关键词
辣椒疫霉/表达序列标签/SSR标记Key words
Phytophthora capsici/expressed sequence tag/SSR marker分类
农业科技引用本文复制引用
王子迎,袁树忠..辣椒疫霉EST-SSRs标记的发掘[J].扬州大学学报（农业与生命科学版）,2009,30(2):69-75,7.基金项目
国家自然科学基金资助项目(30800040) （30800040）
