首页|期刊导航|中国农业科学(英文版)|Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream(Megalobrama terminalis)
中国农业科学(英文版)2004，Vol.3Issue(9)：700-706,7.
Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream(Megalobrama terminalis)
Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream(Megalobrama terminalis)
摘要
Abstract
The insulin-like growth factor Ⅰ(IGF-Ⅰ)gene of triangular bream(Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. The nucleotide sequence analysis showed the Tb IGF-Ⅰ cDNA consisted of 486 nucleotides and encoded 117 amino acids including B,C,A,D and E five domains. Analysis of E-domain indicated that cloned Tb IGF-Ⅰ belonged to IGF-Ⅰ Ea-2 subtype. Identity analysis showed the IGF-Ⅰ nucleotide sequence shared 99.8% homology with bluntnose bream,88.8% with grass Carp,85.8% with common carp; the pre-IGF-Ⅰ amine acid sequence shared 99.4% with bluntnose bream,88.8% with grass carp,85.4% homology with common carp. In the Cyprinus Carpio,the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basic data for the research on Tb germplasm and the development and utilization of biological feed additives.关键词
Triangular bream (Megalobrama terminalis).IGF-Ⅰ.Molecular cloning.Sequence analysisKey words
Triangular bream (Megalobrama terminalis).IGF-Ⅰ.Molecular cloning.Sequence analysis引用本文复制引用
TONG Fu-dan,LIU Hong-yun..Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream(Megalobrama terminalis)[J].中国农业科学(英文版),2004,3(9):700-706,7.基金项目
This research was supported by the Natural Science Foundation of Zhejiang Province,China (300265). （300265）
