中国农业科学（英文版）2005，Vol.4Issue(9)：700-706,7.
Subtype Identification of Avian Influenza Virus on DNA Microarray
Subtype Identification of Avian Influenza Virus on DNA Microarray
摘要
Abstract
We have developed a rapid microarray-based assay for the reliable detection of H5, H7 and H9 subtypes of avian influenza virus (AIV). The strains used in the experiment were A/Goose/Guangdong/1/96 (H5N1), A/African starling/983/79 (H7N1) and A/Turkey/Wiscosin/1/66 (H9N2). The capture DNAs clones which encoding approximate 500-bp avian influenza virus gene fragments obtained by RT-PCR, were spotted on a slide-bound microarray. Cy5-1abeled fluorescent cDNAs,which generated from virus RNA during reverse transcription were hybridized to these capture DNAs. These capture DNAs contained multiple fragments of the hemagglutinin and matrix protein genes of AIV respectively, for subtyping and typing AIV. The arrays were scanned to determine the probe binding sites. The hybridization pattern agreed approximately with the known grid location of each target. The results show that DNA microarray technology provides a useful diagnostic method for AIV.关键词
Avian influenza virus/DNA microarray/Subtype identificationKey words
Avian influenza virus/DNA microarray/Subtype identification分类
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WANG Xiu-rong,YU Kang-zhen,DENG Guo-hua,SHI Rui,LIU Li-ling,QIAO Chuan-ling,BAO Hong-mei,KONG Xian-gang,CHEN Hua-lan..Subtype Identification of Avian Influenza Virus on DNA Microarray[J].中国农业科学（英文版）,2005,4(9):700-706,7.基金项目
We gratefully acknowledge Prof. Tong Guangzhi,Prof. Cao Dianjun, Prof. Wang Xiaomei and Prof.Liu Shengwang for providing us NDV, IBDV, IBV genes as control. This study was supported by Chinese National S&T “10th Five-Year” Plan (2004BA 519A23) （2004BA 519A23）
the National Natural Science Foundation of China (30200201 and 30440009). （30200201 and 30440009）
