水生生物学报2009，Vol.33Issue(4)：702-708,7.DOI:10.3724/SP.J.0000.2009.40702
转基因斑马鱼分析胰岛β-细胞发育情况
ANALYSES OF β-CELL DEVELOPMENT IN TRANSGENIC ZEBRAFISH WITH A CONSTRUCT OF INSULIN PROMOTER AND GREEN FLUORESCENT PROTEIN
摘要
Abstract
With many advantageous features such as small size, high production and fertilization in vitro, zebrafish has become a kind of the model creature for the investigation of vertebrate development and human diseases. So we set up a transgenic zebrafish model to investigate the β-cell development. Firstly, we obtained an INS: GFP construction that contains the zebrafish insulin (INS) promoter and green fluorescent protein (GFP). Secondly, we injected the construction into the cytoplasm of one-cell-stage embryos. Finally, we gained germline INS transgenic zebrafish that displayed highly specific β-cell expression of GFP in both larvae and adult. By following GFP expression, pancreas formation was detected at the 18h post-fertilization in the earliest time points between the second bilateral somites, a group of GFP-positive cells located from ventral to the notochord. From day 1 to 5 post-fertilization, the number of insulin/GFP expressing cells migrated and formed a right organ. Thus, our works demonstrated that the transgenic line provided a convenient and direct experimental tool in analyzing endocrine pancreas development, injury and recovery.关键词
斑马鱼/β-细胞/绿色荧光蛋白/胰岛素Key words
Zebrafish/β-cell/Green Fluorescent Protein (GFP)/Insulin分类
生物科学引用本文复制引用
夏铭,潘雪,李敏燕,邓敏,张勇,金怡,陈漪,王和生,孔德明..转基因斑马鱼分析胰岛β-细胞发育情况[J].水生生物学报,2009,33(4):702-708,7.基金项目
Founds of province(No. 2006109) （No. 2006109）
modernization of traditional Chinese medicine (No. 20075007) （No. 20075007）
