劳动医学2001，Vol.18Issue(2)：70-72,3.
椎间盘体外培养方法及其初步应用的研究
Study on a Culture Method of Intervertebral Disc In Vitro and Its Initial Application
杨进波 1杨磊 1姬文婕 2易桂林 2周宏元 3王辉2
作者信息
- 1. 华中科技大学同济医学院职业医学研究所,
- 2. 华中科技大学同济医学院职业医学研究所，武汉 430030
- 3. 上海海军医学研究所,
- 折叠
摘要
Abstract
［Objective］ To study a new culture method of intervertebral disc with alginate in vitro and using the method to study initially the manifestation and probable mechanism of disc degeneration. ［Method］Lumbar discs separated from Wistar rats were cultured with and without alginate;secondly,nalidixic acid known as a definite degeneration-causing agent was added to the disc cultured with alginate,whereby some biochemical changes were determined successively. ［Results］ With prolongation of culture time,there were no significant changes in contents of collagen,water proportion and the activities of AKP in alginate group (P＞0.05),but they decreased dramatically in the control group (P＜0.01).By rise of nalidixic acid concentration,the contents of HYP and NO,the activities of GSH-Px were found increased significantly. ［Conclusion］ Alginate culture method is better than general method to promote the retention of normal structure and metabolic activities of the intervertebral disc.The increase of the content of HYP is probably a main degenerative manifestation of intervertebral disc,NO and GSH-Px may play important role in the processes.关键词
藻酸盐/椎间盘/离体培养/退变分类
医药卫生引用本文复制引用
杨进波,杨磊,姬文婕,易桂林,周宏元,王辉..椎间盘体外培养方法及其初步应用的研究[J].劳动医学,2001,18(2):70-72,3.
