中国临床康复2004，Vol.8Issue(31)：7072-7074,3.
电针足三里对大鼠脑肠肽含量的影响及神经免疫调控作用
Effect of electro-acupuncture at Tsusanli on the content of braingut peptide in rats and its regulatory function of neuroimmunology
摘要
Abstract
BACKGROUND:Acupuncture can positively regulate both the erythrocytic immunity and T lymphocyte subgroups. But its mechanism still remains to be interpretedOBJECTIVE: To investigate the regulatory role of immune system in the course of electro-acupuncture(EA) in treating relative diseases as well as its mechanism .DESIGN: Randomized and controlled experimental study.SETTING and MATERIALS: Experimental setting: Laboratory of Digestive Diseases, Tangdu Hospital, Fourth Military Medical University of Chinese PLA. Forty rats were randomly divided as normal control group, non-acupoint group, immunosuppressive group, Tsusanli group, and Tsusanli + immunosuppressed group, with 8 rats in each group.INTERVENTIONS:The activity of immunologic response of substance P (ir-SP) and that of vasoactive intestinal peptide (ir-VIP) in rats' pituitary gland and peripheral blood were detected with radioimmunoassay; The changes of T-lymphocytic subgroups were assayed with micro-whole-blood direct immunofluorescence staining and flow cytometry technique. Erythrocytic immunity function was assayed by red blood cell C3b receptor rosette(RBC-C3bRR) and red blood cell immune complexes rosette (RBC-ICR).MAIN OUTCOME MEASURES: Contents of ir-SP and ir-VIP and changes of T-lymphocytic subgroups and the immune function of erythrocyte.RESULTS: The contents of SP and VIP in the peripheral blood and hypophysis [(591.83 ± 142.59) pg/L,(92.05 ±22. 11) pg/L, respectively, in the peripheral blood; (47.00 ±9. 12) pg/L, (308.36 ±34. 16) pg/L, respectively, in the hypophysis ], the percentage of T4 ( CD4 + ) [ (65.55 ± 8. 42) % ],the rosette rate of red blood cell C3b receptor(RBC-C3bRR) [ (15.88 ± 2. 99)％ ],and red blood cell immune complexes rosette (RBC-ICR) [ (12. 14 ± 1.22 )% ]in the Tsusanli group were significantly higher than those in the normal control group [(569.36 ± 8.04) pg/L,(20. 45 ± 6. 74) pg/L, (27.86 ± 7.67) pg/L,(236.71±22.83) pg/L, (43.06±3.14)%, (8.63±2. 13)%, (6.75±2. 32)％,respectively] ( p< 0.01 ). And the CD4 + was positively correlated with the changes of the contents of SP and VIP( r = 0. 744, P<0.05; r = 0.738, P< 0.05; r =0. 822, P<0.05; r =0. 848, P<0.05, respectively), and RBC-C3bRR( r =0. 719, P< 0. 05) . While the contents of SP and VIP in the peripheral blood and hypophysis in the immunosuppressive group decreased, which had statistical difference as compared with the normal control group(P<0. 01). The percentage of T4(CD4 + )cells [(34.50 ±2.52)%]and RBC-C3bRR [ (5.25 ± 1.17)%] were significantly lower than those in the normal control group( P<0. 01, P<0. 05).CONCLUSION: EA can increase the immunity of the body, the mechanism of which may relate with the increase of relative VIP synthesis and release and take effect through these immunotransmitters regulating neuroendocrine-immune network.关键词
电针/穴,足三里/P物质/血管活性肠肽/T淋巴细胞亚群/流式细胞术分类
医药卫生引用本文复制引用
高巍,黄裕新,陈洪,王庆莉..电针足三里对大鼠脑肠肽含量的影响及神经免疫调控作用[J].中国临床康复,2004,8(31):7072-7074,3.基金项目
国家自然科学基金资助项目(39570888) （39570888）
