华南农业大学学报(自然科学版)2001，Vol.22Issue(1)：70-73,4.
昆明小鼠早期胚胎PFK基因转录产物的检测
The Detection of PFK Gene Transcripts by Nested RT-PCR in Early Embryos of Kunming Mouse
摘要
Abstract
Inner and outer primer pair were designed and synthesizedaccording to the cDNA sequence of liver type phosphofructokinase (PFK) of the mouse. A nested reverse transcriptase-polymerase chain reaction (Nested RT-PCR) was used to determine the transcript pattern of mRNA encoding mouse PFK throughout 1-，2-，4-，8-cell and morulae embryos. The results showed that 1-cell to morulae mouse embryos mRNA could be amplified to give an expected band using the inner primer pair on the templates provided by the products of the first PCR using the outer primer pair. These observations indicated that the transcripts of PFK gene existed and that the PFK of early mouse embryos is of the liver type. Glycolysis and tricarboxylic acid cycle (TCA) appear to be the important mode of glucose metabolism in 1-cell to morulae mouse embryos.关键词
昆明小鼠/早期胚胎/6-磷酸果糖激酶/RT-PCR/基因转录分类
生物科学引用本文复制引用
王翀,张守全,杨关福..昆明小鼠早期胚胎PFK基因转录产物的检测[J].华南农业大学学报(自然科学版),2001,22(1):70-73,4.基金项目
国家自然科学基金资助项目( （）
