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西施舌3个自然群体杂合性的研究

王展林 黎中宝 张桂玲 陈锦 赵斌丽 吴宁 林小云

海洋科学2009,Vol.33Issue(7):70-74,5.
海洋科学2009,Vol.33Issue(7):70-74,5.

西施舌3个自然群体杂合性的研究

Heterozygosity in 3 natural stocks of Coelmomactra antiquata (Spengler)

王展林 1黎中宝 1张桂玲 1陈锦 1赵斌丽 1吴宁 1林小云1

作者信息

  • 1. 集美大学,水产学院,集美大学水产生物技术研究所,福建省高校水产科学技术与食品安全重点实验室,福建,厦门,361021
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摘要

Abstract

Heterozygosity was investigated using an assay of vertical slab polyacrylamide gel electrophoresis in three Coelmomactra antiquate stocks from three sites: Beihai (Guangxi Province), Zhanggang (Fujian Province), Qidong (Jiangsu Province). 7 aliozymes were detected, and got 12 allozyme loci, 25 alleles, 4 loci (Sod-1, Mdh-1,Est-1, Est-3) with 1-7 alleles which are polymorphic. The results showed that the genetypic distribution observed at Sod-1, Est-1 in Zhanggang stock and Sod-1 in Beihai stock were corresponded with the Hardy-Wemberg equilibrium (P>0.05). But other loci in the three stocks were significant deviations from Hardy-Weinberg equilibrium (P<0.01). There was heterozygote deficiency existed in Est-1 in Qidong and Zhanggang stock (F>0), and heterozygote excess existed in other loci in the three stocks (F<0). The observed heterozygosity and expected heterozygosity per polymorphic locus per stock indicated that the germplasm resources of three C antiquate stocks were good.

关键词

西施舌(Coelomactra antiquata(spengler))/等位酶/群体/杂合性

Key words

Coelmomactra antiquata (Spengler)/ allozyme/ natural stock/ heterozygosity

分类

农业科技

引用本文复制引用

王展林,黎中宝,张桂玲,陈锦,赵斌丽,吴宁,林小云..西施舌3个自然群体杂合性的研究[J].海洋科学,2009,33(7):70-74,5.

基金项目

福建省自然科学基金项目(B0640010):福建省高等教育新世纪优秀人才支持计划项目(闽教科(2006)35号) (B0640010)

福建省高校水产科学技术与食品安全重点实验室基金项目(2007J202) (2007J202)

集美大学创新团队基金项目(2007A001) (2007A001)

海洋科学

OA北大核心CSCDCSTPCD

1000-3096

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