海洋科学2009,Vol.33Issue(7):70-74,5.
西施舌3个自然群体杂合性的研究
Heterozygosity in 3 natural stocks of Coelmomactra antiquata (Spengler)
摘要
Abstract
Heterozygosity was investigated using an assay of vertical slab polyacrylamide gel electrophoresis in three Coelmomactra antiquate stocks from three sites: Beihai (Guangxi Province), Zhanggang (Fujian Province), Qidong (Jiangsu Province). 7 aliozymes were detected, and got 12 allozyme loci, 25 alleles, 4 loci (Sod-1, Mdh-1,Est-1, Est-3) with 1-7 alleles which are polymorphic. The results showed that the genetypic distribution observed at Sod-1, Est-1 in Zhanggang stock and Sod-1 in Beihai stock were corresponded with the Hardy-Wemberg equilibrium (P>0.05). But other loci in the three stocks were significant deviations from Hardy-Weinberg equilibrium (P<0.01). There was heterozygote deficiency existed in Est-1 in Qidong and Zhanggang stock (F>0), and heterozygote excess existed in other loci in the three stocks (F<0). The observed heterozygosity and expected heterozygosity per polymorphic locus per stock indicated that the germplasm resources of three C antiquate stocks were good.关键词
西施舌(Coelomactra antiquata(spengler))/等位酶/群体/杂合性Key words
Coelmomactra antiquata (Spengler)/ allozyme/ natural stock/ heterozygosity分类
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王展林,黎中宝,张桂玲,陈锦,赵斌丽,吴宁,林小云..西施舌3个自然群体杂合性的研究[J].海洋科学,2009,33(7):70-74,5.基金项目
福建省自然科学基金项目(B0640010):福建省高等教育新世纪优秀人才支持计划项目(闽教科(2006)35号) (B0640010)
福建省高校水产科学技术与食品安全重点实验室基金项目(2007J202) (2007J202)
集美大学创新团队基金项目(2007A001) (2007A001)