中国农业科学（英文版）2005，Vol.4Issue(9)：707-713,7.
Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana
Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana
摘要
Abstract
By screening the worker (Apis cerana cerana) heads cDNA library using a fragment of the mrjp3 gene ofApis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 (AccMRJP3) cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly (A) tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame (ORF) with 1 779 bp encoding 593 amino acids. The un-translated regions (UTR) of the 5' end and 3' end are 46 bp and 160 bp in length,respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.关键词
Apis cerana cerana, MRJP3, Gene cloning, Sequence analysisKey words
Apis cerana cerana, MRJP3, Gene cloning, Sequence analysis分类
农业科技引用本文复制引用
SU Song-kun,ZHNEG Huo-qing,CHEN Sheng-lu,ZHONG Bo-xiong,Stefan Albert..Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana[J].中国农业科学（英文版）,2005,4(9):707-713,7.基金项目
We thank Prof. Dr. Gao Qikang, Institute of Biotechnology, Zhejiang University for his help on technology.We also thank Prof. Miao Yungen for critical reading of the manuscript. This project was supported by the National Natural Science Foundation of China (30200206) and Zhejiang Provincial Natural Science Foundation of China (302113). （30200206）
