中国现代医学杂志2004，Vol.14Issue(8)：7-10,4.
国内ICU耐药黄杆菌携带β-内酰胺酶基因的研究
Study on clinical resistant flavobacterium isolates producing β- iactamases in ICU
摘要
Abstract
Objective: To investigate the type of β - lactamases (bla) produced by three clinical Flavobacterium strains isolated in ICU and the way of the bla being mediated. Methods: Plasmid and chromosome of the strains were extracted using commercial kits. The strains were examined for their producing bla by Nitroeefin disc test. PCR was performed to amplify gene encoding bla in these strains. Results: Nitrocefin disc test showed that the three Flavobacterium strains produced bla. Genetic sequences of PCR products suggested that there were three and six kinds of bla in chromosome and plasmid of the three strains, respectively. Conclusions: It was general and of diversity for the resistant Flavobacterium isolates to carry genes encoding bla. The resistant bla could be mediated by plasmid and chromosome separately or by them together.关键词
ICU/黄杆菌/β-内酰胺酶/基因Key words
ICU/Flavobacterium/β - lactamases/gene分类
医药卫生引用本文复制引用
罗羽,夏培元,王仙园,殷春红..国内ICU耐药黄杆菌携带β-内酰胺酶基因的研究[J].中国现代医学杂志,2004,14(8):7-10,4.基金项目
Supported by Directive Subject of Chinese PLA ( No. 01 L065) （ No. 01 L065）
