国际医药卫生导报2009，Vol.15Issue(7)：7-10,4.DOI:10.3760/cma.j.issn.1007-1245.2009.07.002
SDF-1a/54/KDEL基因电穿孔Molt-4细胞株的转染条件优化
A Study on the Optimization of Electroporation Parameters for SDF-1a/54/KDEL Gene Transfection to Molt-4 Cells in Vitro
摘要
Abstract
Objective To optimize the electroporation parameters for leukemia cell lines Molt-4 cultured in suspension with reconstruction plasmid pEGFP/SDF-1a/54/KDEL (greenfluorescent protein as a reporter gene). Methods pEGFP/SDF-1a/54/KDEL plasmid was transferred into MOLT-4 cells by electroporation with differently experimental conditions such as pulse amplitude, the voltage and the state of cell vitality. The transfection efficiencies were evaluated by flow cytometry (FCM) and fluorescent microscopy. Results The highest electroporation transfection rate (51.2%) was achieved under the condition of voltage 260 V, 950 μF and the vigorous state of MOLT-4 cells. Conclusion Optimized electroporation parameters 260V, 950 μ F and the vigorous state of MOLT-4 cells can improve the transfection efficiency.关键词
电穿孔/SDF-1a/54/KDEL/Molt-4细胞/基因转染Key words
electroporation/SDF-1a/54/KDEL/Molt-4/gene transfection分类
医药卫生引用本文复制引用
陈宏远,芮雯,蔡绍晖..SDF-1a/54/KDEL基因电穿孔Molt-4细胞株的转染条件优化[J].国际医药卫生导报,2009,15(7):7-10,4.基金项目
广东省自然科学基金资助项目(编号:04010459) （编号:04010459）
