寄生虫与医学昆虫学报2000，Vol.ⅦIssue(1)：7-10,4.
套式PCR检测滤纸干血滴中间日疟原虫
DETECTION OF PLASMODIUM VIVAX BY NESTED PCR AMPLIFICATION OF DRIED BLOOD SPOTS ON FILTER PAPERS
摘要
Abstract
Malaria parasites DNA was eluted from dried blood spot on filter paper by Chelex treatment and amplified by PCR to detect low parasitemia of Plasmodium vivax. Sensitivity and specificity of nested PCR assay were confirmed by amplifying a 121 bp DNA fragment of SSUrRNA gene of Plvivax.Density of about 25 parasites per ul of blood can be detected successfully.None of other three human malaria species(Plasmodium falciparum,Plasmodium malariae and Plasmodium ovale)samples was positive.The ease of collection and transport of filter paper specimens combined with the sensitive and specific detection of P.vivax by nested PCR suggest that this method might be a valuable tool for moleculr epidemiological study of P.vivax.关键词
间日疟原虫/套式PCR/滤纸干血滴/ChelexKey words
Plasmodium vivax/nested-PCR/blood filter paper/Chelex分类
生物科学引用本文复制引用
牛春,诸欣平,周蕾,刘强..套式PCR检测滤纸干血滴中间日疟原虫[J].寄生虫与医学昆虫学报,2000,Ⅶ(1):7-10,4.基金项目
国家教委留学回国人员科研启动基金资助项目、北京市教委科技发展基金资助项目. （）
