国际眼科杂志2004，Vol.4Issue(1)：7-11,5.
次声作用后SD大鼠视网膜内皮素-1表达及超微结构改变
ET-1 expression and ultrastructural changes in the retina after exposure to infrasound in rats
摘要
Abstract
AIM: To evaluate the expression of ET-1 and ultrastructural changes in the retina of Sprague-Dawley rats after exposure to infrasound.METHODS: Twenty five male Sprague-Dawley rats were divided into 5 groups, 5 in each group, and 4groups were exposed to infrasound of 16 Hz, 130 dB for 2 hours each day, and 1 group served as control. The exposure time for the 4 groups was respectively 1, 7, 14and 21 days. Then, the rats were sacrificed after intravenous irrigation with lanthanum nitrate (La), and the retina was examined with electron microscopy and immunochemistry with antibodies against ET-1.RESULTS: After infrasonic exposure for 1 day, ET-1 expressed mainly in the endothelium of retinal and choroidal blood vessels. So did it in the control group. After infrasonic exposure for 7 days, it expressed mainly in the retinal pigment epithelial cells, outer nuclear layer and outer plexiform. After exposure for 14 days, its expression was enhanced in the outer nuclear layer, inner plexiform layer and ganglion cell layer. After exposure for 21 days,its expression was weakened in all layers. With exposure going on, the injury in retina gradually extended from the outer to the inner. Under the electronic microscope, La was observed to have filtered into several layers of the retina after 1 day of exposure and it became denser and denser from the 1st to the 21st day except at the 7th day. Meanwhile, the retinal layers showed cellular edema, swollen organelles such as mitochondrion and endoplasmic reticulum, karyopyknosis and edging, cell membrane and karyotheca dilation, rupture and myelin figure.CONCLUSION: The expression of ET-1 in retina is related to the location of infrasonic injury, and ET-1 may have a protective effect on retina against the infrasonic injury.关键词
内皮素/次声/镧/视网膜/免疫组化/电镜/大鼠Key words
endothelin/infrasound/lanthanum/retina/immunohistochemistry/electronic microscopy/rats分类
医药卫生引用本文复制引用
王建洲,惠延年,曹燕,侯旭,邱萍,陈景藻,郭长梅,张自峰..次声作用后SD大鼠视网膜内皮素-1表达及超微结构改变[J].国际眼科杂志,2004,4(1):7-11,5.基金项目
中国国家自然科学基金资助项目(No:39900162) （No:39900162）
