中国药理学与毒理学杂志2006，Vol.20Issue(1)：7-12,6.
吗啡成瘾和应激诱导成瘾复发的大鼠脑内神经甾体水平的变化
Change in brain neurosteroid level of rats in morphine addiction and stress-induced addiction relapse condition
摘要
Abstract
AIM To investigate if morphine addiction and relapse to morphine-seeking is related to the change in neurosteroid levels in the brain of rats. METHODS Rats were injected ip morphine (5 mg·kg-1·d-1, 18:00-20:00) and trained in conditioned place preference (CPP) box, once daily for 10 d. CPP test was performed 24 h after the last training. After discontinuation of training for 7 d for CPP extinction, then intermittent and inescapable foot-shock (FS, 0.5 mA, 0.5 s on, 40 s off, 15 min) was applied to rats as the priming stimuli for relapse. CPP test was performed 2 h after FS. When CPP test finished, rats were decapitated and the levels of neurosteroids were analyzed using gas chromatography/mass spectrometry. RESULTS CPP was established when rats were injected morphine and trained for 10 d. At the same time, the levels of pregnenolone and allopregnanolone in the brain tissues of rats were significantly increased. When CPP was reinstated in morphine-treated rats by FS-stress after 7 d CPP extinction, the levels of dehydroepiandrosterone and dehydroepiandrosterone sulfate were significantly increased. CONCLUSIONThe development of morphine addiction and relapse may be related to endogenous neurosteroids in rat brain tissues.关键词
吗啡/成瘾复发/应激/神经甾体Key words
morphine/addiction relapse/stress/neurosteroids分类
医药卫生引用本文复制引用
闫彩珍,李瑞利,侯艳宁,吴红海,江平..吗啡成瘾和应激诱导成瘾复发的大鼠脑内神经甾体水平的变化[J].中国药理学与毒理学杂志,2006,20(1):7-12,6.基金项目
河北省自然科学基金(C2005000384)The project supported by Natural Science Foundation of Hebei Province (C2005000384) （C2005000384）
