中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(12)：713-718,6.
来源于人膀胱癌细胞株EJ的HSP70负载的DC所激发的特异性CTL细胞的体外应答效应
Cytotoxic T-lymphocytes response in vitro activated by dendritic cells pulsed with heat shock protein 70 derived from human bladder tumor cell lines of EJ
摘要
Abstract
Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lines of E J, were able to induce peptide specific cytotoxic T-lymphocytes (CTL) response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods: The E J-derived HSP70 co-cultured with DC from the healthy volunteers' PBMC, along with the crude lysate (the supematant before HSP70 purification) from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes (SI) and interferon-γ (IFN-γ) were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51Cr releasing test. Results: Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate (the supematant before HSP70 purification) (P < 0.05). DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells (P<0.05). Conclusion: The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor.关键词
heat shock protein 70 (HSP70)/dendritic cells (DC)/cytotoxic T-lymphocytes (CTL)/bladder tumorKey words
heat shock protein 70 (HSP70)/dendritic cells (DC)/cytotoxic T-lymphocytes (CTL)/bladder tumor分类
医药卫生引用本文复制引用
Lingfeng He ,Jianhua Wang ,Xiaofeng Wang ,Xiangjun He ,Zheng Yan ,Kexin Xu ,Kaopeng Guan ,Shukun Hou..来源于人膀胱癌细胞株EJ的HSP70负载的DC所激发的特异性CTL细胞的体外应答效应[J].中德临床肿瘤学杂志（英文版）,2008,7(12):713-718,6.基金项目
a grant from the National Natural Science Foundation of China (No. 3000754). （No. 3000754）
