中华医学杂志（英文版）2001，Vol.114Issue(7)：714-717,4.
胆碱能受体激动剂引起豚鼠离体前庭毛细胞内钙离子浓度升高
Cholinergic agonists increase intracellular calcium concentration in guinea pig vestibular hair cells
韩维举 1张素珍 1韩东一 1姜泗长 1杨伟炎1
作者信息
- 1. 解放军总医院耳鼻喉头颈外科北京 100853
- 折叠
摘要
Abstract
Abstract:Objective To better understand the cholinergic receptors in vestibular hair cells (VHC) and their subtypes, and to investigate the effects of cholinergic agonists on intracellular calcium concentration ([Ca2+]i ) in guinea pig VHCs.Methods VHCs were isolated from guinea pig crista ampullaris by enzymatic and mechanical methods. The effect of cholinergic agonists on [Ca2+]i was examined using laser scanning confocal microscopy and the Ca2+ sensitive dye Fluo-3.Results The results showed that the addition of acetylcholine (ACh) and carbachol (CCh), muscarnic and nicotinic agonists, induced [Ca2+]i increases in all the VHCs, whereas acetylcholine bromide (ACh-Br), a nicotinic agonist, induced the [Ca2+]i increase in only a small percentage of VHCs. The ACh or CCh-induced Ca2+ response could be partially suppressed by atropine. In the presence of 0.1?mmol/L atropine, the amplitudes of ACh or CCh-induced [Ca2+]i responses became significantly smaller than those in atropine free medium (P<0.01). Conclusions The results suggest the existence of cholinergic receptors in guinea pig VHCs. It is the muscarnic agonists rather than nicontic receptors that dominate [Ca2+]i variation. Atropine can suppress muscarnic agonist-induced Ca2+ responses. 关键词
前庭毛细胞/钙/胆碱能受体/共聚焦显微镜Key words
vestibular hair cells/calcium/cholinergic receptors/confocal microscopy分类
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韩维举,张素珍,韩东一,姜泗长,杨伟炎..胆碱能受体激动剂引起豚鼠离体前庭毛细胞内钙离子浓度升高[J].中华医学杂志（英文版）,2001,114(7):714-717,4.
