华西口腔医学杂志2001，Vol.19Issue(2)：71-73,3.
免疫牛乳清对变形链球菌粘附的影响
Effects of Immune Bovine Whey on Sucrose-dependent
Adherence of Streptococcus mutans
樊明文 1陈文霞 1边专 1杜民权 1陈东1
作者信息
摘要
Abstract
The aim of this experiment is to explore the possible anti-carious mechanisms of immune bovine whey.Methods:The immune milk was collected from cows immunized with cell associated glucosyltransferase（GFT） over-expression strain B-29-33 of Streptococcus mutans(S.mutans) MT8148. The control milk was obtained from non-immunized cows. The immune and control bovine whey were prepared by removing fat and casein.The effects of two kinds of bovine whey on sucrose-dependent adherence of S.mutans MT8148 were examined by using TTY media with five different volumes of the immune and the control milk, 100,300,500,700 and 900μl respectively. The adherent rates were determined by reading the optical density. The adherent morphology was observed by using a scanning electron microscope（SEM）. Results: There was no significant difference in adherence of S.mutans MT8148 cells to the smooth glass surface among five doses with those of the control whey. The immune bovine whey inhibited the adherence of S.mutans. The adherent percentage was 84.37% at 100μl and decreased by 58.59% at 900μl.The inhibition was does_dependent. The difference of adherent morphology between the immune and control whey was noticeable at 900μl. The SEM revealed that cells of S.mutans MT8148 with the control whey were covered by large quantities of gelatinous material，but cells with the immune whey were clearly distinguished. Conclusion: The immune whey has inhibitory potential against the sucrose-dependent adherence of S.mutans.关键词
牛奶/变形链球菌/粘附/扫描电镜分类
医药卫生引用本文复制引用
樊明文,陈文霞,边专,杜民权,陈东..免疫牛乳清对变形链球菌粘附的影响[J].华西口腔医学杂志,2001,19(2):71-73,3.
