中国水稻科学2000，Vol.14Issue(2)：71-78,8.
用籼/籼交重组自交系群体构建的分子遗传图谱及其与籼/粳交群体的分子图谱的比较
A Molecular Map Based on an Indica/Indica Recombinant Inbred Population and Its Comparison with an Existing Map Derived from Indica/Japonica Cross in Rice
摘要
Abstract
With the eventual purpose of precisely locating quantitative trait loci (QTLs) in indica rice, a molecular linkage map was constructed based on an indica × indica derived recombinant inbred (RI) population consisting of 131 (F6∶7) lines. The map consists of 160 RFLP markers detected by 113 rice and 28 wheat probes and 78 AFLP markers revealed by five PstⅠ/MseⅠ primer combinations. The heterozygosity rate of the population was close to the expectation, indicating that the population had a normal genetic structure. Segregation distortion was observed in six chromosomal regions, suggesting that abnormal segregation could happen even in an intra-subspecies population. The map covers a length of 1435.8 cM, with an average distance of 6.38 cM between adjacent markers. A set of RFLP landmarks generated by the Japanese Rice Genome Program (RGP) was applied to allow comparison between our map and the RGP map. Both maps have nearly the same total map length regarding the coverage by common markers. Nine of 12 chromosomes showed complete linkage conservation in the two maps and rice chromosome 1 represented strong linearity to wheat group 3 chromosomes. Distinct organization between the two maps was evident. Four small inversions in chromosome arms 1S, 1L, 4L and 8L and nineteen additional loci mapping to all twelve chromosomes except chromosomes 5 and 6 were detected. Four regions on chromosome arms 2S, 7S, 10L and 11S exhibited no, or at least very low polymorphism resulting in obvious distal deficiencies in these regions of the indica map. The conservation of duplicated fragments between chromosome 11 and 12 was not detected in the indica map. Chromosome rearrangement involving in genome evolution in O. sativa is discussed.关键词
图谱/限制性片段长度多态性/扩增片段长度多态性/水稻/重组自交系/数量性状基因位点Key words
mapping/RFLP/AFLP/rice/recombinant inbred line/quantitative trait loci分类
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..用籼/籼交重组自交系群体构建的分子遗传图谱及其与籼/粳交群体的分子图谱的比较[J].中国水稻科学,2000,14(2):71-78,8.基金项目
This work was funded in part by the Royal Society of UK, the Rockefeller Foundation, the Youth Science Foundation of High Technology Research and Development Programme of China, and the National Natural Science Foundation of China (39670411). （39670411）
