中国组织工程研究与临床康复2008，Vol.12Issue(36)：7183-7186,4.
碱性氨基酸对丙交酯/乙交酯共聚物体外降解时集酸程度的调节
Effect of basic amino acid on acid accumulation during poly(D,L-lactide-co-glycol ide)degradation in vitro○
周伟 1刘彬 2戴小珍 2周婵 3蔡绍哲 3宋国立3
作者信息
- 1. 西南大学校医院,重庆市400715
- 2. 西南大学生命科学学院,重庆市400715
- 3. 重庆大学生物工程学院,重庆市400030
- 折叠
摘要
Abstract
BACKGROUND:Poly(D,L-lactide-co-glycolide)(PLGA)may produce lactic acid and glycolic acid when PLGA degrades,thus leading to the acid accumulation and inducing the inflammatory reaction locally.OBJECTIVE:To investigate the effect of lysine,histidine and arginine on regulating the acid accumulation of PLGA copolymer during the degradation.DESIGN:Repeated measurement and experiment.TIME AND SETTING:The experiments were performed in the College of Bioengineering.Chongqing University from July 2006 to August 2007.MATERIALS:PILGA(80:20)was produced by Sigma(USA);Lysine,histidine and arginine(purlty>99%)were purchased from Sigma(USA);Chitosan(deacetylation degree:85%)was purchased from Chengdu Kelong Chemical Reagent Factory;Algin(viscosity:1.05-1.15)was purchased from Tianiin Damao Chemical Reagent Factory.METHODS:Lysine,histidine and arginine were added into PLGA with the proportion of 5%(w/v)and 10%(w/v)respectively.The resultant film sample was put into a bottle with tri-distilled-water for 2-month degradation at 37℃.The pH value of degradation solution was detected by pH meter;Each film sample was taken out and lyophilized 12 hours to get its dry weight and calculate mass loss ratio.Each variety of the solution was sampled three specimens,the average pH value,average initial weight and average finial weight of these three specimens were taken as the indices at each sampling time point,respectively.Accordingly,the regulation effect of basic amino acid was comparexl with that of algin,chitosan and NaHCO3.MAIN OUTCOME MEASURES:The changes of pH value of degradation solution;the mass loss ratio of the composite.RESULTS:Each basic additive could relieve the acid accumulation,among them,NaHCO3 was the strongest,while algin and chitosan showed a lowest capacity,basic amino acid was moderate;The suitable regulation effect could be achieved at a level of 5%lysine.CONCLUSION:Basic amino acid can effectively regulate the acid accumulation after PLGA degrades,and the optimal concentration of lysine is 5%.关键词
丙交酯/乙交酯共聚物/降解/碱性氨基酸/pH/壳聚糖分类
医药卫生引用本文复制引用
周伟,刘彬,戴小珍,周婵,蔡绍哲,宋国立..碱性氨基酸对丙交酯/乙交酯共聚物体外降解时集酸程度的调节[J].中国组织工程研究与临床康复,2008,12(36):7183-7186,4.
