中国农业科学(英文版)2003，Vol.2Issue(7)：722-727,6.
A Heat Shock-Switchable Gene Expression System
A Heat Shock-Switchable Gene Expression System
摘要
Abstract
The expression of β-D-glueuronidase (Gus) gene under the control of soybean heat shock protein(HSP) promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heatshock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly duringthe recovery phase (21℃) after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric as-says indicated that maximal Gus activity after heat shock treatment could reach 5 - 6 times higher than that be-fore heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock pro-moter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used tocontrol expression of gene of interest in transgenic plants.关键词
Tobacco/Promoter/Heat shock/InducibleKey words
Tobacco/Promoter/Heat shock/Inducible引用本文复制引用
CHEN Ming,WANG Li-xia,PENG Xiang-lei,ZHONG Jin,XU Hui-jun,LIN Zhong-ping..A Heat Shock-Switchable Gene Expression System[J].中国农业科学(英文版),2003,2(7):722-727,6.基金项目
This work was supported by the National High Tech R&D Program of China (863 Program,2002AA2244011) and the National Special Program for Research and Industrialization of Transgenic Plants, China (J2000-B-021). （863 Program,2002AA2244011）
